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Enzymes
are chemical substances produced in the living organism.
They are marvellous organic catalysts which are essential
to life as they control all the chemical reactions that
take place in a living system. Enzymes are part of all
living cells, including those of plants and animals.
The term enzyme, which literally means in yeast,
was coined following the demonstration of catalytic
properties of yeast and yeast juices. Although enzymes
are produced in the living cell, they are not dependent
upon the vital processes of the cell and work outside the
cell. Certain enzymes of yeast, for instance, when
expressed from the yeast cells are capable of exerting
their usual effect, that is, the conversion of sugar to
alcohol.
A striking feature of enzymes is that while they enter
into chemical reaction, they remain intact in the
process. They however, act with maximum efficiency at a
certain temperature. Lowering the temperature below or
raising it above this level slows the reaction. A high
degree of heat, that is above 60 o C,
permanently destroys their action.
It has been estimated that there are over 20,000 enzymes
in the human body. This estimate is based on the number
of bodily processes that seem to require action. However,
so far only about 1,000 enzymes have been identified. But
their great role in nutrition and other living processes
has been firmly established. They are protein molecules
made up of chains of amino acids. They play a vital role
and work more efficiently than any reagent concocted by
chemists. Thus for instance, a chemist can separate
proteins into their component amino acids by boiling them
at 166 o C for over 18 hours in a strong solution of
hydrochloric acid, but the enzymes of the small
intestines can do so in less than three hours at body
temperature in a neutral medium.
A feature which distinguishes enzymes from inorganic
catalysts is that they are absolutely specific in their
actions. This means that a particular enzyme can cause
reactions involving only a particular type of substance
or a group of closely related substances. The substance
on which the enzyme acts is known as
"substrate". The specificity of an enzyme is,
however, related to the formation of the enzyme-substrate
complex which requires that the appropriate groupings of
both substrate and enzyme should be in correct relative
position. The substrate must fit the enzyme like a key
fits its lock.
Enzymes which are used in the cells which make them are
called intracellular enzymes. Enzymes which are produced
in cells which secrete them to other parts of the body
are known as extracellular enzymes. Digestive juices are
an example of the latter type.Nomenclature
There are few
enzymes whose names have been established by long usage
such as ptyalin, pepsin, trypsin and erepsin. Apart from
these, enzymes are usually named by adding the suffixes
to the main part of the name of the substrate upon which
they act. Thus amylases act upon starch (amylum), lac-
tase acts upon lactose, lipases act upon lipids, maltase
acts upon maltose and protesses act upon lipids, maltase
acts upon maltose and protesses act upon proteins. There
are, however, several enzymes which act upon many
substances in different ways. These enzymes are named by
their functions rather than substrates. Thus, an enzyme
which causes deaminations is called a deaminase and
oxidising enzyme an oxidase.
Some enzymes work efficiently only if some other specific
substance is present in addition to substrate. This other
substance is known as an "activator" or a
"conenzyme" . "Acti- vators" are
usually inorganic ions. They increase the activity of a
complete enzyme and may take part in the formation of the
enzyme-substrate complex. Many of the conenzymes are
related to vitamins. This explains why vitamin
deficiencies profoundly alter metabolism. Thus, for
instance, thiamine, as thiamine pyrophosphate, functions
as a conenzyme in at least 14 enzymes systems.
Conenzymes, like enzymes, are being continuously
regenerated in the cells.
Enzymes play a decisive role in the digestion of food as
they are responsible for the chemical changes which the
food undergoes during digestion. The chemical changes
comprise the breaking up of the large molecules of
carbohydrates, fats and proteins into smaller ones or
conversion of complex substances into simple ones which
can be absorbed by the intestines. They also control the
numerous reactions by which these simple substances are
utilized in the body for building up new tissues and
producing energy. The enzymes themselves are not broken
down or changed in the process. They remain as powerful
at the end of a reaction as they were at the beginning.
Moreover, very small amounts can convert large amounts of
material. They are thus true catalysts.
The process of digestion begins in the mouth. The saliva
in the moth, besides helping to masticate the food,
carries an enzyme called ptyalin which begins the
chemical action of digestion. It initiates the catabolism
(breakdown) of carbohydrates by converting starches into
simple sugars. This explains the need for thorough
mastication of starchy food in the mouth. If this is not
done the ptyalin cannot carry out its functions as it is
active in an alkaline, neutral or slightly acid medium
and is inactivated by the highly acid gastric juices in
the stomach.
Although enzymatic action starts while food is being
chewed, digestion moves into high gear only when the
chewed food has passed the esophagus and reached the
stomach. While the physical action of peristalsis churns
and kneads solid food into a semi-solid amorphous mixture
called chyme, this mixture undergoes chemical changes
initiated by gastric juices secreted by the walls of the
stomach. These juices include mucus for lubricating the
stomach, hydrochloric acid and gastric juice. The enzyme
or active principle of the gastric juice is pepsin. This
enzyme in combination with hydrochloric acid starts the
breakdown of proteins into absorbable amino acids called
polypeptides. An additional enzyme, rennin, plays an
important role in the stomach of the infant. It curdles
milk and allows the pepsin to work upon it. The gastric
juice has no effect upon starches or fats.
When the chyme leaves the stomach and enters the small
intestine through the pylorus - the lower escape valve,
it still contains much food which is in the form of raw
material not yet ready for absorption in the body.
Digestion is completed inside the small intestine by
several juices. From liver comes a liquid called bile
which converts fat globules into a smooth emulsion.
The pancreas contributes various enzymes which continue
the breakdown of proteins, help to divide starch into
sugars and work with bile in digesting fats. The small
intestine itself secretes enzymes from its inner wall to
complete the reactions. When all the enzymes have done
their work, the food is digested and rendered fit for
absorption by the system.The following table briefly
summarises the chemical digestion of carbohydrates, fats
and proteins by various enzymes :
| Source of Enzyme |
Enzyme |
Substrate |
Products |
| Mouth
Salivary glands |
Salivary
amylase (ptyalin) |
Starch
|
Dextrins
and maltose |
| Stomach
|
Gastric
protease |
|
|
| Gastric
mucosa |
pepsin
|
Proteins
|
Polypeptides |
| |
rennin
|
casein
|
insoluble
casein |
| |
Gastric
lipase |
Short
chain & medium chain triglycerides |
Fatty
acids and glycerol |
| Small
intestine |
Pancreatic
Proteases, trypsin chymotrypsin carboxypeptidases |
Proteins
and polypeptides |
Smaller
-polypeptides & amino acids |
| |
Panocreatic
lipase (steapsin) |
Fats
|
Mono
and diglycerides, fatty acids and glycerol |
| |
Pancreatic
amylase (amylopsin) |
Amylose
& amylopectin |
Maltose,
maltotriose & a-limit dextrins |
| Intestinal
mucosa Brushborder |
Intestinal
peptidases aminopeptideses dipeptideses |
Polypeptides
Dipeptides |
Smaller
polypeptides & amino acids |
| |
Intestinal
saccharidases a-dextrinase (isomaltase) |
a-limit
dextrins |
Glucose |
| |
Sucrase
|
Sucrase
|
Glucose
& fructose |
| |
Maltase
|
Maltose
|
Glucose(2
molecules) |
| |
Lactase
|
Lactose
|
Glucose
& galactose |
Enzymes form part of
the food we eat. Raw foods contain enzymes in abundance ;
cooking, pasteurising, pickling, smoking and other
processings denature enzymes. It is, therefore, essential
to include in our diet, substantial amount of raw foods
in the form of fruits, raw salads and sprouts. Studies
have revealed that the body without sufficient raw
materials from raw foods, may tire and produce fewer
enzymes year after year. This may lead to wearing out of
body processes and consequent worn-out looks.
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